LX-1

Zellname	Beschreibung	Bestell-Nr.	Einheit	Preis, Euro
LX-1	Humane Lungenkarzinom Zell-Linie	300268	cryovial	375,00
LX-1	Humane Lungenkarzinom Zell-Linie	330268	vital	465,00

Designation:	LX-1
Depositor:	CLS
Organism:	Homo sapiens (human)
Ethnicity:	Caucasian
Age/Stage:	48 years
Gender:	male
Tissue:	lung
Morphology:	epithelial
Celltype:	carcinoma, undifferentiated
Growth Properties:	On collagen-coated flasks adherent; clusters.
Description:	Established in vitro from the primary lung carcinoma of a 48 year-old male, the primary lung tumor was an oat cell carcinoma.
Culture Medium:	Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, 1% non- essential amino acids (NEAA) and 10% fetal bovine serum.
Subculturing:	Remove medium, add fresh 0,025% trypsin/0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Use collagen-coated (rat tail) cell culture flasks to improve attachment.
Split Ratio:	A ratio of 1:2 to 1:6 is recommended
Fluid Renewal:	1 to 2 times weekly
Freeze Medium:	CM-1 (CLS ∙ Cell Lines Service)
Sterility:	Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:	1
Tumorigenic:	yes, in nude mice
Reverse Transcriptase:	negative
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