LX-289
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
LX-289 |
Humane Lunge Adenokarzinom Zell-Linie |
300269 |
cryovial |
265,00 |
LX-289 |
Humane Lunge Adenokarzinom Zell-Linie |
330269 |
vital |
335,00 |
| Designation: | LX-289 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 62 years |
| Gender: | Male |
| Tissue: | Lung |
| Morphology: | Epithelial |
| Celltype: | Adenocarcinoma |
| Growth Properties: | Monolayer |
| Description: | The LX-289 was etablished in vitro from the primary lung adenocarcinoma of a 62 year-old male. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle`s BSS supplemented with L-glutamine, non- essential amino acids and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for Mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Antigen Expression: | Cytokeratine 8 positive , Cytokeratine 18 positive, Desmoplakin and Vimentin |
| Reverse Transcriptase: | negative |
References: not published | |
