NCI-H128
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NCI-H128 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
300181 |
cryovial |
265,00 |
NCI-H128 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
330181 |
vital |
335,00 |
| Designation: | NCI-H128 |
| Depositor: | Gazdar |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Black |
| Age/Stage: | 60 years |
| Gender: | Male |
| Tissue: | Lung; pleural effusion |
| Morphology: | floating aggregates |
| Celltype: | small cell lung carcinoma |
| Growth Properties: | suspension |
| Description: | This cell line is aneuploid. Will form colonies in soft agar. It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. NCI-H128 cells do well on a rotary shaker flask at 70 to 80 rpm at 37°C. It is normal for cultures of this line to have fairly large amounts of cell debris. |
| Culture Medium: | RPMI 1640 media supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms tumors with typical small cell carcinoma histology |
| Karyotype: | There are 2 distinct aneuploid peaks, both have characteristic 3p deletion. |
| Isoenzymes: | G6PD, A; PGM3, 1; PGM1, 1; ES-D, 1; Me-2, 1; GLO-1, 1-2; AK-1, 1; Phenotype Frequency Product: 0.0078 |
References: Gazdar AF et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. Cuttitta F et al. Monoclonal antibodies that demonstrate specificity for several types of human lung cancer. Proc Natl Acad Sci USA 78: 4591-5, 1981. Little CD et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. Tong AW et al. Characterization of two human small cell lung carcinoma-reactive monoclonal antibodies generated by a novel immunization approach. Cancer Res 44: 4987-92, 1984. Carney DN et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. Gazdar AF et al. Charaterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological and growth properties. Cancer Res. 45: 2924-2930, 1985. Curt GA et al. Determinants of the sensivity of human small-cell lung cancer cell lines to methotrexate. J Clin Invest 76: 1323-9, 1985. Falzon M et al. Xenobiotic-metabolizing enzyme activity in human non-small-cell drived lung cancer cell lines. Biochem Pharmacol 35: 563-8, 1986. Hensel CH et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. Yokoyama T et al. Combination of vitamin K2 plus imatinib mesylate enhances induction of apoptosis in small cell lung cancer cell lines. Int J Oncol 26: 33-40, 2005. | |
