NCI-H146
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NCI-H146 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
300182 |
cryovial |
265,00 |
NCI-H146 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
330182 |
vital |
335,00 |
| Designation: | NCI-H146 |
| Depositor: | Schlegel |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 59 years |
| Gender: | male |
| Tissue: | Lung (pleural effusion) |
| Morphology: | epithelial |
| Celltype: | small cell lung carcinoma |
| Growth Properties: | aggregates in suspension |
| Description: | The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from the pleural fluid of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide. |
| Culture Medium: | RPMI 1640 medium supplemented with 10% fetal bovine serum (the serum content may be reduced to 5%) |
| Subculturing: | The cells should be sub cultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes; forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen |
| Oncogene: | myc +; c-myb +;c- raf +; Ha-ras +; Ki-ras + ; N-ras +; v-fms +; fes - |
| Karyotype: | This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations. |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6,9.3 TPOX: 8,11 vWA: 14,16 D3S1358: 11 D21S11: 30 D18S51: 15,17 Penta E: 12 Penta D: 13 D8S1179: 12,14 FGA: 23 CLS ∙ Cell Lines Service, 2010. |
| Receptors Expressed: | insulin-like growth factor II receptor (IGF II) |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 1; Phenotype Frequency Product = 0.0009 |
References: Banks-Schlegel SP et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res 45: 1187-1197, 1985. Carney DN et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res 45: 2913-2923, 1985. Curt GA et al. Determinants of the sensitivity of human small-cell lung cancer cell lines to methotrexate. J Clin Invest 76: 1323-9, 1985. Roth KA et al. Small cell carcinoma cell lines contain opioid peptides and receptors. Cancer 57: 769-73, 1986. Robinson BW et al. Natural killer (NK)-resistant human lung cancer cells are lysed by recombinant interleukin-2-activated NK cells. Cell Immunol 106: 215-22, 1987. Kiefer PE et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res 47: 6236-6242, 1987. Bepler G et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. Pancrazio JJ et al. Voltage-dependent ion channels in small-cell lung cancer cells. Cancer Res 49: 5901-6, 1989. Hensel CH et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res 50: 3067-3072, 1990. Verbeeck MA et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. Schardt C et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp Cell Res 204: 22-29, 1993. | |
