NCI-H69
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NCI-H69 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
300185 |
cryovial |
265,00 |
NCI-H69 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
330185 |
vital |
335,00 |
| Designation: | NCI-H69 |
| Depositor: | Little |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 55 years |
| Gender: | male |
| Tissue: | small cell lung carcinoma; lung |
| Morphology: | floating aggregates |
| Growth Properties: | suspension |
| Description: | This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate.The cells stain positively for cytokeratins. The line can be adapted to grow in shaker flask or spinner flask systems.The N-myc gene is amplified, and there is expression of the mRNA and protein.C-myc mRNA, but not protein, is expressed at a low level.There is expression of c- myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms tumors with typical small cell carcinoma histology |
| Oncogene: | myc +; myb +; fes +, fms +; raf +; ras + |
| Karyotype: | aneuploid, with 3p deletion; range = 40 to 73 |
| Receptors Expressed: | insulin-like growth factor II receptor (IGF II) |
| Isoenzymes: | G6PD, B; PGM1, 2; PGM3, 1; ES-D, 2; Me-2, 1; AK-1, 1; GLO-1, 1-2; Phenotype Frequency Product: 0.00006 |
References: Gazdar AF et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res 40: 3502-7, 1980. Little CD et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-6, 1983. Carney DN et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res 45: 2913-23, 1985. Gazdar AF et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res 45: 2924-30, 1985. Kiefer PE et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res 47: 6236-42, 1987. Broers JL et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J Cell Sci 91: 91-108, 1988. Bepler G et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. Hensel CH et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res 50: 3067-72, 1990. Schardt C et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp Cell Res 204: 22-9, 1993. Rygaard K et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int J Cancer 54: 144-52, 1993. Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res 57: 5356-9, 1997. Geiger T et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des 13: 35-45, 1998. Moody TW et al. Camptothecin-somatostatin conjugates inhibit the growth of small cell lung cancer cells. Peptides 26: 1560-6, 2005. Niiya M et al. Amurubicinol-induced eotaxin-3 expression in human NCI-H69 small cell lung carcinoma cells. Oncol Rep 15: 571-6, 2006. | |
