NCI-H82
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NCI-H82 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
300442 |
cryovial |
265,00 |
NCI-H82 |
Humane Lungenkarzinom (kleinzellig) Zell-Linie |
330442 |
vital |
335,00 |
| Designation: | NCI-H82 |
| Depositor: | Little |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 40 years |
| Gender: | male |
| Tissue: | carcinoma, small cell lung cancer; lung; pleural effusion |
| Morphology: | epithelial |
| Growth Properties: | aggregates in suspension; the cells grow in very Large aggregates, and the aggregates are the only viable cell population |
| Description: | The NCI-H82 cell line was derived by A.F. Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung. The morphology of the original tumor was not characteristic of SCLC. The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase. It does not have detectable levels of L-DOPA decarboxylase or bombesin. The cells produce an abnormally sized p53 mRNA (3.7 kb). C-myc DNA sequences are amplified about 25 fold, and there is a 24 fold increase in c-myc RNA relative to normal cells. The cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern. The cells stain positively for neurofilaments and vimentin. There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. |
| Culture Medium: | RPMI 1640 media supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes; forms transplantable tumors with non-typical SCLC histology in nude mice |
| Oncogene: | myc +; myb -; raf +; ras +; fms +; fes + |
| Karyotype: | This is a near triploid human cell line. The modal chromosome number is 58, occurring at 44% with polyploidy at 3%. Marker chromosomes der(1)t(1;709p13;p11), t(13q;?HSR;15q) and der(190t(19;?)(q13.4;?) were common to most cells. There were two distinct subpopulations readily distinguished by karyotype. Besides uniform changes in the numbers of copies of some normal chromosomes, one population had der(3)t(3;20)(p11;p11?), t(3q19p), i(7q) and a minute chromosome of unknown origin. The other had t(1q17p), del(1)(q21), der(3)t(3;7)(p12;q11) plus two other markers. Each cell had two copies of a normal X chromosome. The Y chromosome was not detected in Q banded preparations. |
| Receptors Expressed: | insulin-like growth factor II receptor (IGF II); atrial natriuretic peptide (ANP) |
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1; Phenotype Frequency Product = 0.0082 |
References: Gazdar AF et al. Levels of creatine kinase and its BB isoenzyme in lung cancer specimens and cultures. Cancer Res 41: 2773-7, 1981. Little CD et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-6, 1983. Carney DN et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res 45: 2913-23, 1985. Gazdar AF et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer havingdistinctive biochemical, morphological, and growth properties. Cancer Res 45: 2924-30, 1985. Takahashi T et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-4, 1989. Hensel CH et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res 50: 3067-72, 1990. Ohsaki Y et al. Human small cell lung cancer cell lines express functional atrial natriuretic peptide receptors. Cancer Res 53: 3165-71, 1993. Schardt C et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp Cell Res 204: 22-29, 1993. Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res 57: 5356-9, 1997. Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-31, 1997. Shinoda C et al. Doxorubicin induces expression of mutidrug resistance-associated protein 1 in human small cell lung cancer cell lines by the c-jun N-terminal kinase pathway. Int J Cancer 117: 21-31, 2005. Grigorova M et al. Chromosome abnormalities in 10 lung cancer cell lines of the NCI-H series analyzed with spectral karyotyping. Cancer Genet Cytogenet 162: 1-9, 2005. | |
