SK-LU-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-LU-1 |
Humane Lungen Adenokarzinom (GIII) Zell-Linie |
300335 |
cryovial |
265,00 |
SK-LU-1 |
Humane Lungen Adenokarzinom (GIII) Zell-Linie |
330335 |
vital |
335,00 |
| Designation: | SK-LU-1 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 60 years |
| Gender: | female |
| Tissue: | Lung |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma (grade III) |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamine, 1% nonessential amino acids, 1 mM sodium pyruvate and 10% fetal bovine serum |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immunotolerant rats and nu-nu mice |
| Karyotype: | The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation. |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 10 D16S539: 8 D5S818: 11 D7S820: 9 THO1: 7 TPOX: 8,10 vWA: 16,17 D3S1358: 18 D21S11: 29,30.2 D18S51: 18 Penta E: 5 Penta D: 10,13 D8S1179: 10 FGA: 21,22 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41 |
| Isoenzymes: | Me-2, 1; PGM3, 1; PGM1, 2; ES-D, 2; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.00003 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. ShimizuK et al. Three human transforming genes are related to the viral ras oncogenes. Proc Natl Acad Sci USA 80: 2112-6, 1983. Zhang X et al. Sodium 4-phenylbutyrate induces apoptosis of human lung carcinoma cells through activating JNK pathway. J Cell Biochem 93: 819-29, 2004. Chin SF et al. Telomerase- and alternative telomere lenghtening-independent telomere stabilization in a metastasis-derived human non-small cell lung cancer cell line: effect of ectopic hTERT. Cancer Res 66: 3584-92, 2006. | |
