SK-MES-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-MES-1 |
Humane Lungenkarzinom Zell-Linie |
300339 |
cryovial |
265,00 |
SK-MES-1 |
Humane Lungenkarzinom Zell-Linie |
330339 |
vital |
335,00 |
| Designation: | SK-MES-1 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 65 years |
| Gender: | male |
| Tissue: | squamous carcinoma; lung; pleural effusion |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and sodium pyruvate, 90%; fetal bovine serum,10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. |
| Antigen Expression: | Blood Type O; Rh+; HLA A3, Aw30, B7, B27 |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0132 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. Blobel GA et al. Cytokeratins in normal lung and lung carcinomas. I. Adenocarcinomas, squamous cell carcinomas and cultured cell lines. Virchows Arch B Cell Pathol Incl Mol Pathol 45: 407-29, 1984. Merry S et al. Circumvention of drug resistance in human non-small cell lung cancer in vitro by verapamil. Br J Cancer 56: 401-5, 1987. Li B et al. Activation of the proapoptotic death receptor DR5 by oligometric peptide and antibody agonists. J Mol Biol 361: 522-36, 2006. Chien W et al. Suppression of cell proliferation and signaling transduction by connective tissue growth factor in non-small cell lung cancer cells. Mol Cancer Res 4: 591-8, 2006. | |
