WI-38 VA-13 subline 2RA
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
WI-38 VA-13 subline 2RA |
Humane Lungenzellen (embryonal) |
300421 |
cryovial |
265,00 |
WI-38 VA-13 subline 2RA |
Humane Lungenzellen (embryonal) |
330421 |
vital |
335,00 |
| Designation: | WI-38 VA-13 subline 2RA |
| Depositor: | Girardi |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 3 months gestation |
| Gender: | Female |
| Tissue: | Lung |
| Morphology: | Epithelial |
| Celltype: | Fibroblast |
| Growth Properties: | Adherent |
| Description: | This cell line is a SV40-transformed variant of the Wi38 cell line. |
| Culture Medium: | Minimum essential medium (Eagle) in Hanks' BSS with non-essential amino acids, 1 mM pyruvic acid and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA(versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution for 3 to 5 minutes, remove trypsin and let culture stand at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:10 is recommended |
| Fluid Renewal: | Twice per week |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 2 (contain Papovavirus) |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | herpes simplex; vesicular stomatitis (Indiana); poliovirus 2 |
References: Jensen F et al. Autologous and homologous impantation of humen cells transformed in vitro by simian virus 40. J.Natl. Cancer Inst. 32: 917-937, 1964. Girardi AJ et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. Wang S and Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. Cerone MA, Londono-Vallejo JA, Bacchetti S. Telomere maintenance by telomerase and by recombination can coexist in human cells. Hum Mol Genet 10(18): 1945-1952, 2001. Tao L et al. Immortalization of human Wi38 cells is associated with differential activation of the c-myc origins. J Cell Biochem 82(3): 522-534, 2001. | |
