AGS
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
AGS |
Humane Magen Adenokarzinom Zell-Linie |
300408 |
cryovial |
265,00 |
AGS |
Humane Magen Adenokarzinom Zell-Linie |
330408 |
vital |
335,00 |
| Designation: | AGS |
| Depositor: | Barranco |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 54 years |
| Gender: | female |
| Tissue: | stomach |
| Morphology: | epithelial |
| Celltype: | gastric adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The AGS cell line was derived from fragments of a biopsy specimen of an untreated human adenocarcinoma of the stomach. This cell line may release Parainfluenzavirus Type 5 (formerly known as Simian Virus 5). The virus interferes with Interferon-signalling within the cell line by degradation of STAT1. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse monolayer with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.020% EDTA solution and let the culture incubate at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Doubling Time: | 20 hrs |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma (DAPI), bacteria and fungi were negative |
| Biosafety Level: | 2 |
| Tumorigenic: | yes, in athymic BALB/c mice |
| Karyotype: | modal number = 47; range = 39 to 92 |
References: Barranco SC et al. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res 43: 1703-9, 1983. Barranco SC et al. Heterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs. Invest New Drugs 1: 117-27, 1983. Segal ED et al. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc Natl Acad Sci USA 93: 1259-64, 1996. Rieder G et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect Immun 65: 3622-30, 1997. Huang J et al. Contribution of C/EBP proteins to Epstein-Barr virus lytic gene expression and replication in epithelial cells. J Virol 80: 1098-1109, 2006. Thors C et al. Thomsen-Friedenreich oncofetal antigen in Schistosoma mansoni: localization and immunogenicity in experimental mouse infection. Parasitology 132: 73-81, 2006. Riles WL et al. Resveratrol engages selective apoptotic signals in gastric adenocarcinoma cells. World J Gastroenterol 12: 5628-34, 2006. Chaput C et al. Role of AmiA in the morphological transition of Helicobacter pylori and in immune escape. PloS Pathog 2: e97, 2006. Young DF, Carlos TS, Hagmaier K, Fan L und Randall RE. AGS and other tissue culture cells can unknowingly be persistently infected with PIV5; a virus that blocks interferon signalling by degrading STAT1. Virology 365(1):238-40, 2007. | |
