CLS-145
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CLS-145 |
Humane Magen Adenokarzinom Zell-Linie |
300180 |
cryovial |
315,00 |
CLS-145 |
Humane Magen Adenokarzinom Zell-Linie |
330180 |
vital |
395,00 |
| Designation: | CLS-145 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | European |
| Age/Stage: | 70 years |
| Gender: | female |
| Tissue: | Stomach |
| Morphology: | epithelial |
| Celltype: | gastric papillary adenocarcinoma; pars cardiaca |
| Growth Properties: | monolayer |
| Description: | The CLS-145 cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. MAP-Test negative, PAS positive |
| Culture Medium: | Ham's F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse the monolayer with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.05% EDTA solution in PBS without calcium and magnesium and let the culture incubate at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Doubling Time: | 36 |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice (Rhythm 20-30 days) |
References: not published | |
