HGC-27
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HGC-27 |
Humane Magenkarzinom Zell-Linie |
300436 |
cryovial |
265,00 |
HGC-27 |
Humane Magenkarzinom Zell-Linie |
330436 |
vital |
335,00 |
 "Zoom (42KB)")
| Designation: | HGC-27 |
| Depositor: | Riken Gene Bank |
| Organism: | Homo sapiens (human) |
| Tissue: | stomach |
| Morphology: | Epithelial; polygonal or short spindle-shaped |
| Celltype: | gastric carcinoma |
| Growth Properties: | monolyaer |
| Description: | The HGC-27 cell line was established by culture of the metastatic lymph node from a gastric cancer patient diagnosed histological as undifferentiated carcinoma. |
| Culture Medium: | Iscove's modified Dulbecco's medium supplemented with 2 mM L-glutamin and 20% fetal bovine serum. Altenatively, DMEM:F12 supplemented with 2 mM L-glutamin and 10% fetal bovine serum can be used. |
| Subculturing: | split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10 000 cells cm2 using trypsin/EDTA |
| Fluid Renewal: | every 2 to 3 days |
| Doubling Time: | 17 hrs |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes |
| Modal Number: | Mode of 109 and 110 chromosomes |
References: Akagi T, Kimoto T. Human cell line (HGC-27) derived from the metastatic lymph node of gastric cancer. Acta Med Okayama 30(3):215-219, 1976. Messa C et al. Inhibitory effct of 17beta-estradiol on growth and the polyamine metabolism of a human gastric carcinoma cell line (HGC-27). Scand J Gastroenterol 34(1): 79-84, 1999. Pessina A et al. In vitro sensitivity of human gastric cancer cells (HGC-27) to Heliobacter pylori cytotoxin. Scand J Gastroenterol 38(12): 1228-1234, 2003. Wang MX, Ren LM. Growth inhibitory effect and apoptosis induced by extracellular ATP and adenosine on human gastric carcinoma cells: involvement of intracellular uptake of adenosine. Acta Pharmacol Sin 27(8): 1085-1092, 2006. | |
