KATO-III
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
KATO-III |
Humane Magenkarzinom Zell-Linie |
300381 |
cryovial |
265,00 |
KATO-III |
Humane Magenkarzinom Zell-Linie |
330381 |
vital |
335,00 |
| Designation: | KATO-III |
| Depositor: | Sekiguchi |
| Organism: | Human |
| Ethnicity: | Asian |
| Age/Stage: | 55 years old |
| Gender: | male |
| Tissue: | Stomach (pleural effusion). From metastatic site: supraclavicular and axillary lymph nodes and Douglas cul-de-sac |
| Morphology: | spherical |
| Celltype: | gastric carcinoma |
| Growth Properties: | suspension/monolayer upon long-term cultivation |
| Culture Medium: | Ham's F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Subculture by diluting aliquots in new flasks containing fresh medium. Dislodge adherent cells by scraping. |
| Split Ratio: | A ratio of 1:2 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Doubling Time: | 36 hrs (Sekiguchi M, 1978) |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes; in cheek pouches of anti thymocyte serum treated hamsters; not tumorigenic in nude mice |
| Karyotype: | The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected (Sekiguchi 1978). |
| Antigen Expression: | Blood Type B; Rh+ |
| Isoenzymes: | PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0742 |
References: Sekiguchi M et al. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn J Exp Med 48: 61-68, 1978. Yachi A et al. Human gastric cancer-associated antigens and carcinoembryonic antigen detected by monoclonal antibodies. Ann NY Acad Sci 417: 158-68, 1983. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res 52: 2460-3, 1992. Rieder G et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect Immun 65: 3622-30, 1997. Masamune A et al. Rebamipide inhibits ceramide-induced Interleukin-8 production in KATO-III human gastric cancer cells. J Pharmacol Exp Therapeutics 298: 485-92, 2001. Hibasami H et al. Glycyrrhetic acid (a metabolic substance and aglycon of glycyrrhizin) induces apoptosis in human hepatoma, promyelotic leukemia and stomach cancer cells. Int J Mol Med 17: 215-9, 2006. Matsunobu T et al. Expression of keratinocyte growth factor receptor correlates with expansive growth and early stage of gastric cancer. Int J Oncol 28: 307-14, 2006. Pierzchalski P et al. The mechanism of heat-shock protein 70 gene expression abolition in gastric epithelium caused by Heliobacter pylori infection. Heliobacter 11: 69-104, 2006. | |
