DU-145
Cell name |
Description |
Order no. |
Units |
Price, Euro |
|---|---|---|---|---|
DU-145 |
Human Prostate Carcinoma cell line |
300168 |
cryovial |
265,00 |
DU-145 |
Human Prostate Carcinoma cell line |
330168 |
vital |
335,00 |
| Designation: | DU 145 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 69 years |
| Gender: | male |
| Tissue: | prostate |
| Morphology: | epithelial |
| Celltype: | carcinoma; from metastatic site: brain |
| Growth Properties: | monolayer |
| Description: | DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia. The line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments and desmosomes. Many mitochondria, well developed Golgi and heterogenous lysosomes. The cells do not express prostate antigen. |
| Culture Medium: | Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms adenocarcinoma (grade II) consistent with prostatic primary |
| Karyotype: | (P75) hypotriploid to tetraploid with abnormalities including breaks, dicentrics, minutes and large telocentric marker |
| Antigen Expression: | Blood Type O; Rh+ |
| Isoenzymes: | Me-2, 1-2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0041 |
References: Mickey DD, Stone KR, Wunderli H, Mickey GH, Vollmer RT, Paulson DF. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. Human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. Papsidero LD, Kuriyama M, Wang MC, Horoszewicz J, Leong SS, Valenzuela L, Murphy GP, Chu TM. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. Pollack MS, Heagney SD, Livingston PO, Fogh J. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst.66: 1003-1012, 1981. Sheng S, Carey J, Seftor EA, Dias L, Hendrix MJ, Sager R. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. Carter RE, Feldman AR, Coyle JT. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. Robinson D, He F, Pretlow T, Kung JH. A tyrosine kinase profile of prostate carcinoma. Proc. Natl. Acad. Sci. USA 93: 5958-5962, 1996. Su ZZ, Lin J, Shen R, Fisher PE, Goldstein NI, Fisher PB. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. Zhu X, Kumar R, Mandal M, Sharma N, Sharma HW, Dhingra U, Sokolski JA, Hsiao R, Narayama R. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. Boffa LC, Morris PL, Carpaneto EM, Loissaint M, Allfrey VG. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. Hu SX, Ji W, Zhou Y, Logothetis C, Xu HJ. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. Nupponen NN, Hyytinen ER, Kallioniemi AH, Visakorpi T. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. | |
