LNCaP
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
LNCaP |
Humane Prostata Karzinom Zell-Linie |
300265 |
cryovial |
265,00 |
LNCaP |
Humane Prostata Karzinom Zell-Linie |
330265 |
vital |
335,00 |
| Designation: | LNCaP |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 50 years |
| Gender: | male |
| Tissue: | prostate (from metastatic site: left supraclavicular lymph node) |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | clusters; lightly adherent |
| Description: | This cell line was established from a metastatic lesion of human prostatic adenocarcinoma. |
| Culture Medium: | Minimum Essential medium (Eagle) supplemented with 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse the monolayer with 0.02% EDTA/PBS. Add fresh 0.025% trypsin/0.02% EDTA/PBS solution and incubate until the cells detach. Add complete medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | twice weekly |
| Doubling Time: | 60 hr |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | pseudodiploid male; seven marker chromosomes; modal number = 46; range = 33 to 91 |
| Modal Number: | 76 to 91 |
| Receptors Expressed: | androgen; estrogen |
| Products: | human prostatic acid phosphatase; prostate specific antigen |
References: Horoszewicz JS et al. The LNCaP cell line - a new model for studies on human prostatic carcinoma. Prog Clin Biol Res 37 : 115-32, 1980. Papsidero LD et al. Prostate antigen : a marker for human prostate epithelial cells. J Natl Cancer Inst 66 : 37-42, 1981. Horoszewicz JS et al. LNCaP model of human prostatic carcinoma. Cancer Res 43: 1809-18, 1983. Murphy, G.P., ed., Models for prostate cancer. 37, New York:Liss; 1980:pp. 115-132. Gibas Z et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet Cytogenet 11: 399-404, 1984. Boffa LC et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J Biol Chem 271: 13228-33, 1996. Umekita Y et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc Natl Acad Sci USA 93: 11802-7, 1996. Hu SX et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res 57: 3339-43, 1997. Liao J et al. Extracellular calcium as a candidate mediator of prostate cancer skeletal metastasis. Cancer Res 66: 9065-73, 2006. Sivaprasad U et al. Differential efficacy of 3-hydroxy-3-methylglutaryl CoA reductase inhibitors on the cell cycle of prostate cancer cells. Mol Cancer Ther 5: 2310-6, 2006. | |
