293 (HEK-293)
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
293 (HEK-293) |
Humane embryonale Nieren Zell-Linie |
300192 |
cryovial |
265,00 |
293 (HEK-293) |
Humane embryonale Nieren Zell-Linie |
330192 |
vital |
335,00 |
MSDS_HEK293
MSDS_293 _HEK-293_.pdf (24KB)
MSDS_293 _HEK-293_.pdf
ZKBS - Stellungnahme zu HEK293
 "Zoom (35KB)")
| Designation: | 293 (HEK-293) |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Synonym(s): | HEK-293 (human embryonic kidney) |
| Age/Stage: | fetus |
| Tissue: | Kidney (transformed with adenovirus 5 DNA) |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells express the transforming gene of adenovirus 5. Adenovirus 5 DNA from both the right and left ends of the viral genome are present. The line is excellent for titrating human adenoviruses. The cell line does not adhere to the substrate when left at room temperature for any length of time. The cells will reattach to the flask over a period of several days in culture at 37°C. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. |
| Culture Medium: | DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium to deactivate trypsin, centrifuge, add fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 According to the GenTSV §5 Abs. 2 i.V.m.Anhang Teil B, Teil A II, and the statement of the ZKBS (Central committee for Biological Safety, Germany), the cell line 293 is categorized to Biosafety level 1. The 293 cell line is in accordance with an established human cell line, which contains parts of a viral genome but does not release infectious virus particles. |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12,14 S16S539: 9,13 D5S818: 8,9 D7S820: 11,12 TH01: 7,9,3 TPOX: 11 vWA: 16,19 D3S1358: 15,17 D21S11: 28,30.2 D18S51: 18 Penta E: 7,15 Penta D: 9,10 D8S1179: 12,14 FGA: 23 CLS – Cell Lines Service 2010. |
| Receptors Expressed: | vitronectin |
| Applications: | Transfection |
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