769-P
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
769-P |
Humane Nierenkarzinom Zell-Linie |
300106 |
cryovial |
265,00 |
769-P |
Humane Nierenkarzinom Zell-Linie |
330106 |
vital |
335,00 |
 "Zoom (9KB)")
| Designation: | 769-P |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 63 years |
| Gender: | female |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | renal cell adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | This line was derived from a primary clear cell adenocarcinoma. The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes. They can be grown in soft agar. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Remove trypsin by centrifugation, add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:12 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Doubling Time: | 35 |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immuno-suppressed hamsters and nude mice |
| Karyotype: | hypodiploid; modal number = 45; a large submetacentric chromosome was present in all cells |
| DNA Profile (STR): | Amelogenin: X, X CSF1PO: 11, 12 D13S317: 10, 14 D16S539: 9, 13 D5S818: 12 D7S820: 10, 11 THO1: 6, 9.3 TPOX: 8, 11 vWA: 18, 18 D3S1358: 16, 16 D21S11: 28, 30 D18S51: 14, 17 Penta E: 7, 18 Penta D: 12, 16 D8S1179: 12, 16 FGA: 20, 22 CLS ∙ Cell Lines Service, 2010. |
References: Williams RD et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-7, 1976. Williams RD et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-86, 1978. Griffith TS et al. Induction and regulation of tumor necrosis factor-related apoptosis-inducing ligand/Apo-2 ligand-mediated apoptosis in renal cell arcinoma. Cancer Res 62: 3093-9, 2002. Vila MR et al. Hepatitis A virus receptor blocks cell differentiation and is overexpressed in clear cell renal cell carcinoma. Kidney Int 65: 1761-73, 2004. Pandha H et al. Antitumor effects of aminobisphosphates on renal cell carcinoma cells lines. J Urol 176: 2255-61, 2006. | |
