A-498
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-498 |
Humane Nierenkarzinom Zell-Linie |
300113 |
cryovial |
265,00 |
A-498 |
Humane Nierenkarzinom Zell-Linie |
330113 |
vital |
335,00 |
| Designation: | A-498 |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 52 years |
| Gender: | Female |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The A-498 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | MEM Eagle supplemented with 2 mM L-glutamine, 1mM sodium-pyruvate, NEAA and 10% fetal bovine serum. Incubate at 37°C / 5% CO2. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA (versene) solution. Add 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes; in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice |
| Karyotype: | Bimodal; tetrapoidy |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 12 D16S539: 12 D5S818: 11,13 D7S820: 11,12 THO1: 6,9.3 TPOX: 8,11 vWA: 18 D3S1358: 15 D21S11: 28,32 D18S51: 17 Penta E: 10,14 Penta D: 9,14 D8S1179: 13,15 FGA: 18,20 CLS ∙ Cell Lines Service, 2010. |
| Isoenzymes: | PGM3, 1; PGM1, 1-2; ES-D, 2; Me-2, 1; AK-1, 1; GLO-1, 2; G6PD, B; |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One-hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res 52: 2460-3, 1992. Griffith TS et al. Induction and regulation of tumor necrosis factor-related apoptosis-inducing ligand/Apo-2 ligand-mediated apoptosis in renal cell arcinoma. Cancer Res 62: 3093-9, 2002. Pfoertner S et al. Cellular retinoic acid binding protein I: expression and functional influence in renal cell carcinoma. Tumour Biol 26: 313-23, 2005. Deegan C et al. In vitro anti-tumour effect of 1,10-phenanthroline-5,6-dione (phendione), [Cu(phendione)3](ClO4)2.4H2O and [Ag(phendione)2]ClO4 using human epithelial cell lines. Chem Biol Interact 164: 115-25, 2006. Lock EA et al. Caspase-dependent and -independent induction of phosphatidylserine externalization during apoptosis in human renal carcinoma Cak(1)-1 and A-498 cells. Toxicology 229: 79-90, 2007. | |
