A-704
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-704 |
Humane Nieren Adenokarzinom Zell-Linie |
300217 |
cryovial |
265,00 |
A-704 |
Humane Nieren Adenokarzinom Zell-Linie |
330217 |
vital |
335,00 |
| Designation: | A-704 |
| Depositor: | Giard |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 78 years |
| Gender: | male |
| Tissue: | adenocarcinoma; kidney |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and sodium pyruvate, 85%; fetal bovine serum, 15% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | (P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 7,8 D13S317: 8 D16S539: 12,13 D5S818: 10,11 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 14,18 D3S1358: 15 D21S11: 28,32 D18S51: 16,17 Penta E: 8,17 Penta D: 2.2,11 D8S1179: 13,15 FGA: 22,23 CLS ∙ Cell Lines Service, 2010. |
| Isoenzymes: | Me-2, 1; PGM3, 1-2; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Fogh J. Cultivation, charcterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Metge K et al. MDR1 expression in human leukemia-lymphoma cell lines. Leukemia 7: 896-7, 1993. Zorn U et al. Induction of cytokines and cytotoxicity against tumor cells by Newcastle disease virus. Cancer Biother 9: 225-35, 1994. Ishitoya S et al. Verotoxin induces rapid elimination of human renal tumor xenografts in SCID mice. J Urol 171: 1309-13, 2004. | |
