ACHN
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
ACHN |
Humane Nieren Adenokarzinom Zell-Linie |
300117 |
cryovial |
265,00 |
ACHN |
Humane Nieren Adenokarzinom Zell-Linie |
330117 |
vital |
335,00 |
| Designation: | ACHN |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 22 years |
| Gender: | male |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | Growth is inhibited by human interferon. |
| Culture Medium: | Eagle's MEM supplemented with 2 mM L-glutamine, 1% non-essential amino acids, 1 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11 D13S317: 12 D16S539: 12,13 D5S818: 12 D7S820: 9,11 THO1: 8 TPOX: 8,11 vWA: 16,17 D3S1358: 17 D21S11: 30 D18S51: 16 Penta E: 16 Penta D: 12 D8S1179: 12 FGA: 22 CLS ∙ Cell Lines Service, 2010. |
| Receptors Expressed: | CAIX negative (FACS analysis). |
References: Collen D et al. Coronary thrombolysis in dogs with intravenously administered human pro-urokinase. Circulation 72: 384-8, 1985. Nobuhara M et al. The inhibition of neoplastic cell proliferation with human natural tumor necrosis factor. Jpn J Cancer Res 78: 193-201, 1987. Sullivan CM et al. Identification of constitutive and gamma-interferon- and interleukin 4-regulated proteins in the human renal carcinoma cell line ACHN. Cancer Res 57: 1137-43, 1997. Kress S and Greenlee WF. Cell-specific regulation of human CYP1A1 and CYP1B1 genes. Cancer Res 57: 1264-9, 1997. Griffith TS et al. Induction and regulation of tumor necrosis factor-related apoptosis-inducing ligand/Apo-2 ligand-mediated apoptosis in renal cell arcinoma. Cancer Res 62: 3093-9, 2002. Miyata Y et al. Presence of phosphorylated hepatocyte growth factor receptor/c-Met is associated with tumor progression and survival in patients with conventional renal cell carcinoma. Clin Cancer Res 12: 4876-81, 2006. Horiguchi A et al. Leptin promotes invasiveness of murine renal cancer cells via extracellular signal-regulated kinases and rho dependent pathway. J Urol 176: 1636-41, 2006. Morais C et al. Pyrrolidine dithiocarbamate exerts anti-proliferative and pro-apoptotic effects in renal cell carcinoma cell lines. Nephrol Dial Transplant 21: 3377-88, 2006. | |
