RC-124
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RC-124 |
Humane Niere Zell-Linie |
300251 |
cryovial |
375,00 |
RC-124 |
Humane Niere Zell-Linie |
330251 |
vital |
465,00 |
| Designation: | RC-124 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 63 years |
| Gender: | male |
| Tissue: | kidney |
| Morphology: | epithelial, |
| Growth Properties: | monolayer |
| Description: | Established from the kidney of a 63-years-old-male, 1998 |
| Culture Medium: | McCoy's 5a medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | 2n=46 |
| Products: | cytokeratine 8, 18, 19, vimentin |
References: not published | |
