RCC-FG1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RCC-FG1 |
Humane Niere Klarzell-Karzinom Zell-Linie |
300248 |
cryovial |
375,00 |
RCC-FG1 |
Humane Niere Klarzell-Karzinom Zell-Linie |
330248 |
vital |
465,00 |
| Designation: | RCC-FG1 (KTCTL-26) |
| Depositor: | Prof. Pomer; CLS, 1999 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 69 years |
| Gender: | male |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | clear cell carcinoma pT2a, M1/ GII |
| Growth Properties: | monolayer |
| Description: | Established from the kidney clear cell carcinoma pT2a, M1/GII of a 69-years-old-male, 1999; PAS positive. The cells show high expression of P-170 glycoprotein ( Lutz et al. 2005 ) |
| Culture Medium: | McCoy's 5a medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 5-10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests were negative for mycoplasma (DAPI) |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Immunology: | HLA-A2 negative; cytokeratine 8+,18+, 19+; vimentin+ |
References: Frank MH, Pomer S. Interferon alpha2b differentially affects proliferation of two human renal cell carcinoma cell lines differing in the P-glycoprotein-associated multidrug-resistant phenotype. J Cancer Res Clin Oncol. 125 (2):117-20, 1999. Lutz NW, Franks SE, Frank MH, Pomer S. Hull WE. Investigation of multidrug resistance in cultured human renal cell carcinoma cells by 31P-NMR spectroscopy and treatment survival assays. MAGMA.18(3):144-61, 2005. | |
