RCC-FG2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RCC-FG2 |
Humane Niere Klarzell-Karzinom Zell-Linie |
300249 |
cryovial |
375,00 |
RCC-FG2 |
Humane Niere Klarzell-Karzinom Zell-Linie |
330249 |
vital |
465,00 |
| Designation: | RCC-FG2 (KTCTL-26A) |
| Depositor: | Pomer S. / CLS, 1999 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 69 years |
| Gender: | male |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | clear cell carcinoma pT2a, Nx, M1/GII |
| Growth Properties: | monolayer |
| Description: | Established from the kidney clear cell carcinoma of a 69-years-old-male, pT2a, Nx, M1/ GII; 1999; HLA-A2 positive; PAS positive, G250 positive |
| Culture Medium: | RPMI 1640 medium supplemented with 5.1 ml L-glutamine (200 mM) and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA(versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS · Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | Near-diploid range; partial loss of chromosome 3p (ie, 3p14), trisomy of chromosome 7 |
| Immunology: | cytokeratin 8+,18+, 19+; vimentin |
References: Hogernann I et al. Cytogenetic and growth factor gene analysis of a renal carcinoma cell line. Cancer Genet Cytogenet 78(2): 175-180, 1994. Kopf-Maier P et al. The alkylator treosulfan shows activity towards human renal-cell carcinoma in vivo and in vitro. In Vivo 12(3): 275-288, 1998. Kopf-Maier P et al. Antitumor activity of titanocene dichloride in xenografted human renal-cell carcinoma. Anticancer Res 19(1A): 493-504, 1999. | |
