RCC-HS
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RCC-HS |
Humane Niere Klarzell-Karzinom Zell-Linie |
300250 |
cryovial |
375,00 |
RCC-HS |
Humane Niere Klarzell-Karzinom Zell-Linie |
330250 |
vital |
465,00 |
| Designation: | RCC-HS (KTCTL-30) |
| Depositor: | CLS, 1999 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 59 years old |
| Gender: | female |
| Tissue: | Kidney |
| Morphology: | epithelial |
| Celltype: | clear cell carcinoma pT1, NO, Mx/GI |
| Growth Properties: | monolayer |
| Description: | Established from the kidney clear cell carcinoma pT1, No, Mx/GI of a 59-years-old female in 1999. |
| Culture Medium: | RPMI 1640 medium supplemented with 5.1 ml L-glutamine (200 mM) and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA(versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution, remove trypsin and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days. |
| Split Ratio: | A ratio of 1:2 to 1:3is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 11 D16S539: 13 D5S818: 12 D7S820: 11,12 THO1: 7 TPOX: 8,10 vWA: 14,15 D3S1358: 15 D21S11: 30,31 D18S51: 15,18 Penta E: 10,12 Penta D: 9,11 D8S1179: 10,14 FGA: 23,25 CLS ∙ Cell Lines Service, 2010. |
References: Kopf-Maier P. The alkylator treosulfan shows activity towards human renal-cell carcinoma in vivo and in vitro. In Vivo 12(3): 275-288, 1998. Kopf-Maier P. Antitumor activity of titanocene dichloride in xenografted human renal-cell carcinoma. Anticancer Res 19(1A):493-504, 1999. Zigeuner RE et al. Immunomagnetic cell enrichment detects more disseminated cancer cells than immunocytochemistry in vitro. J Urol 164(5):1834-1837, 2000. Westphal G et al. Detection of NGF-receptors TrkA and p75NTR in human tumor cell lines and effect of NGF on the growth characteristics of the UT-7/EPO cell line. J Exp Clin Cancer Res 21(2): 255-267, 2002. Zigeuner RE et al. Isolation of circulating cancer cells from whole blood by immunomagnetic cell enrichment and unenriched immunocytochemistry in vitro. J Urol 169(2): 701-705, 2003. | |
