RCC-MF
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RCC-MF |
Humane Niere Klarzell-Karzinom Zell-Linie |
300245 |
cryovial |
375,00 |
RCC-MF |
Humane Niere Klarzell-Karzinom Zell-Linie |
330245 |
vital |
465,00 |
| Designation: | RCC-MF (KTCTL-1M ) |
| Depositor: | Pomer S. / CLS, 1999 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 63 years |
| Gender: | male |
| Tissue: | kidney |
| Morphology: | epithelial |
| Celltype: | clear cell carcinoma |
| Growth Properties: | monolayer |
| Description: | Established from the kidney clear cell carcinoma pT2, N1, Mx/ GII-III (lung-metastasis) of a 63 years old male in 1997. |
| Culture Medium: | RPMI-1640 medium supplemented with 5.1 ml L-glutamine (200 mM) and 10% fetal bovine serum |
| Subculturing: | Remove medium and rinse with 0.02% EDTA(versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Immunology: | cytokeratine 8+,18+,19+; vimentin |
| Receptors Expressed: | G250 positive (CAIX positive, as confirmed by FACS analysis). |
References: Kopf-Maier P. The alkylator treosulfan shows activity towards human renal-cell carcinoma in vivo and in vitro. In Vivo 12(3):275-288, 1998. Kopf-Maier P. Antitumor activity of titanocene dichloride in xenografted human renal-cell carcinoma. Anticancer Res 19(1A):493-504, 1999. | |
