RCC-PR

Zellname

Beschreibung

Bestell-Nr.

Einheit

Preis, Euro

RCC-PR

Humane Niere Klarzell-Karzinom Zell-Linie

300267

cryovial

375,00

RCC-PR

Humane Niere Klarzell-Karzinom Zell-Linie

330267

vital

465,00



Designation:RCC-PR
Depositor:CLS, 2001
Organism:Homo sapiens (human)
Ethnicity:Caucasian / European
Age/Stage:81 years
Gender:female
Tissue:kidney
Morphology:epithelial
Celltype:carcinoma
Growth Properties:monolayer
Description:Established from kidney carcinoma
Culture Medium:RPMI 1640 medium supplemented with 2 mM L-glutamin and 10% fetal bovine serum
Subculturing:Remove medium and rinse with 0.02% EDTA(versene) solution. Add fresh 0.025% trypsin/0.02% EDTA(versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 5 days.
Split Ratio:A ratio of 1:2 to 1:3 is recommended
Fluid Renewal:1 to 2 times weekly
Freeze Medium:CM-1, Cell Lines Service
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1


References:

not published

 

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