SK-NEP-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SK-NEP-1 |
Humane Niere (Wilm's Tumor) Zell-Linie |
300341 |
cryovial |
265,00 |
SK-NEP-1 |
Humane Niere (Wilm's Tumor) Zell-Linie |
330341 |
vital |
335,00 |
| Designation: | SK-NEP-1 |
| Depositor: | Fogh |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 25 years |
| Gender: | female |
| Tissue: | Wilms' tumor; pleural effusion |
| Morphology: | epithelial |
| Growth Properties: | suspension |
| Description: | Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles. |
| Culture Medium: | RPMI 1640 media supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Establish new cultures at 1 X 10 exp5 cells/ml and maintain at between 10 exp5 and 10 exp6 cells/ml. Replace spent media by centrifuging, aspirate supernatant, and resuspend the cell pellet in fresh cell culture medium. |
| Fluid Renewal: | every 2 to 4 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor |
| Karyotype: | (P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,11 vWA: 15,19 D3S1358: 14,15 D21S11: 29,31 D18S51: 15,17 Penta E: 7,18 Penta D: 11,12 D8S1179: 12 FGA: 24 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A; Rh+ |
| Isoenzymes: | PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0029 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Fogh J. Cultivation, characterization, and indentification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Orkin SH et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-4, 1984. Pinthus JH et al. Inhibition of Wilms tumor xenograft progression by halofuginone os accompanied by activation of WT-1 gene expression. J Urol 174: 1527-31, 2005. Abramson LP et al. Pigment epithelium-derived factor targets endothelial and epithelial cells in Wilms' tumor. J Pediatr Surg 41: 1351-6, 2006. | |
