CaKi-2

Zellname	Beschreibung	Bestell-Nr.	Einheit	Preis, Euro
CaKi-2	Humane Niere Klarzell-Karzinom Zell-Linie	300140	cryovial	265,00
CaKi-2	Humane Niere Klarzell-Karzinom Zell-Linie	330140	vital	335,00

Designation:	Caki-2
Depositor:	Fogh
Organism:	Homo sapiens (human)
Ethnicity:	Caucasian
Age/Stage:	69 years
Gender:	male
Tissue:	Kidney
Morphology:	epithelial
Celltype:	clear cell carcinoma;
Growth Properties:	monolayer
Description:	Ultrastructural features include microvilli and microfilaments. Few mitochondria, lysosomes or lipid droplets. Frequent multilamellar bodies. No virus particles.
Culture Medium:	McCoy's 5A medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum
Subculturing:	Remove medium and rinse with fresh 0.02% EDTA. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, collect the cells and dispense into new flasks. Subculture every 6 to 8 days.
Split Ratio:	A ratio of 1:3 to 1:6 is recommended
Fluid Renewal:	2 to 3 times weekly
Freeze Medium:	CM-1 (CLS · Cell Lines Service)
Sterility:	Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:	1
Tumorigenic:	yes, in nude mice; forms clear cell carcinoma
Karyotype:	(P8) hypopentaploid to hypohexaploid (+A2, +A3, +B, +C, +D, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, minutes, breaks, and large subtelocentric markers
Antigen Expression:	Blood Type A; Rh-
Isoenzymes:	Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0511
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