NCI-H295R
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
NCI-H295R |
Humane Nebennieren Karzinom Zell-Linie |
300483 |
cryovial |
265,00 |
NCI-H295R |
Humane Nebennieren Karzinom Zell-Linie |
330483 |
vital |
335,00 |
| Designation: | NCI-H295R |
| Depositor: | Rainey |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Black |
| Age/Stage: | 48 years |
| Gender: | female |
| Tissue: | adrenal gland |
| Morphology: | epithelial |
| Celltype: | adrenocortical carcinoma |
| Growth Properties: | monolayer |
| Description: | NCI-H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line established by A.F. Gazdar and associates (1990) from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. While the original cells grew in suspension, the adapted cells were selected to grow in a monolayer. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions. |
| Culture Medium: | A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 15 mM HEPES, 0.00625 mg/ml insulin, 0.00625 mg/ml transferrin, 6.25 ng/ml selenium, 1.25 mg/ml bovine serum albumin, and 0.00535 mg/ml linoleic acid and 2.5% Nu-Serum I. (see also CLS-order-No. 820402 / MG-42) |
| Subculturing: | Remove medium and rinse with fresh 0.025% trypsin/0.02% EDTA solution. Add fresh trypsin/EDTA solution (1 to 2 ml) and let the culture sit at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A sub cultivation ratio of 1:3 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1 (CLS Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO:10,12 D13S317: 13 D16S539: 11 D5S818: 12 D7S820: 9,12 TH01: 9.3 TPOX: 8 vWA: 17,18 D3S1358: 15,16 D21S11: 32.2 D18S51: 17 Penta E: 5,12 Penta D: 8 D8S1179: 13 FGA: 19.2, 24 |
| Products: | Aldosterone; cortisol; C19 steroids |
References: Gazdar AF et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res 50: 5488-96, 1990. Cobb VJ et al. Forskolin treatment directs steroid production towards the androgen pathway in the NCI-H295R adrenocortical tumour cell line. Endocr Res 22: 545-50, 1996. Brand C et al. Smad is involved in the intracellular signaling pathways that mediate the inhibitory effects of transforming growth factor-beta n StAR expression. Biochem Biophys Res Commun 253: 780-5, 1998. Willenberg HS et al. Corticotropin-releasing hormone receptor expression on normal and tumorous human adrenocortical cells. Neuroendocrinology 82: 274-81, 2005. Ansurudeen I et al. Vascular-adrenal niche—endothelial cell-mediated sensitization of human adrenocortical cells to angiotensin II. Horm Metab Res 38: 476-80, 2006. | |
