SW-13
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-13 |
Humane Nebennieren Adenokarzinom Zell-Linie |
300349 |
cryovial |
265,00 |
SW-13 |
Humane Nebennieren Adenokarzinom Zell-Linie |
330349 |
vital |
335,00 |
| Designation: | SW-13 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 55 years |
| Gender: | female |
| Morphology: | epithelial |
| Celltype: | small cell carcinoma; adrenal gland |
| Growth Properties: | monolayer |
| Description: | Electron microscopic studies show many bulb gap junctions (BGJ). |
| Culture Medium: | Leibovitz's L-15 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 2 to 3 minutes, remove trypsin and let culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | vesicular stomatitis (Indiana); poliovirus 1 |
References: Lasfargues EY et al. Cultivation of human breast carcinomas. J Natl Cancer Inst 21: 1131-47, 1958. Johnson RG et al. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-9, 1971. Pinto da Silva P et al. Gap junctions in normal and transformed fibroblasts in culture. Exp Cell Res 71: 393-401, 1972. Leibovitz A et al. New human cancer cell culture lines. I.SW-13, small-cell carcinoma of the adrenal cortex. J Natl Cancer Inst 51: 691-7, 1973. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Estes Ii NR et al. siRNA mediated knockdown of fibroblast growth factor receptors 1 or 3 inhibits FGF.induced anchorage-independent clonogenicity but does not affect MAPK activation. Oncol Rep 15: 1407-16, 2006. Zhang L et al. Versatile reporter systems show that transactivation by human T-cell leukemia virus type 1 Tax occurs independently of chromatin remodeling factor BRG1. J Virol 80: 7459-68, 2006. | |
