AsPC-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
AsPC-1 |
Humane Pankreas Adenokarzinom Zell-Linie |
300158 |
cryovial |
265,00 |
AsPC-1 |
Humane Pankreas Adenokarzinom Zell-Linie |
330158 |
vital |
335,00 |
| Designation: | AsPC-1 |
| Depositor: | Tan |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 62 years of age |
| Gender: | female |
| Tissue: | Pancreas (ascites) |
| Celltype: | adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas. |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine, 1 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA (versene) solution. Add 0.025% trypsin/0.02% versene solution and let the culture sit at 37°C until the cells detach (control under the microscope). Add fresh, FBS containing medium, resuspend in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Products: | carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin |
References: Tan MH et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J Natl Cancer Inst 67: 563-9, 1981. Chen WH et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. Loor R et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin Lab Med 2: 567-78, 1982. Tan MH and Chu TM. Charcterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumour Biol 6: 89-98, 1985. Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res 50: 2997-3001, 1990. Nuevemann D et al. Stable expression of temperature-sensitive p53 : a suitable model to study wild-type p53 function in pancreatic carcinoma cells. Oncol Rep 16: 575-9, 2006. El-Rayes BF et al. Potentation of the effect of erlotinib by genistein in pancreatic cancer: the role of Akt and nuclear factor-kappaB. Cancer Res 66: 10553-9, 2006. | |
