Capan-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Capan-1 |
Humane Pankreas Adenokarzinom Zell-Linie |
300143 |
cryovial |
265,00 |
Capan-1 |
Humane Pankreas Adenokarzinom Zell-Linie |
330143 |
vital |
335,00 |
| Designation: | Capan-1 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 40 years |
| Gender: | male |
| Tissue: | Pancreas (from metastatic site: liver) |
| Morphology: | epithelial |
| Celltype: | adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The cells will slough off of the growth surface if they become too heavy. Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secretes gastric type mucin. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM Glutamine and 10% foetal bovine serum. |
| Subculturing: | Remove medium and rinse with EDTA (versene) solution. Add fresh 0.025% trypsin / EDTA (versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium, centrifuge to remove trypsin, resuspend in fresh medium and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms adenocarcinoma consistent with pancreatic duct carcinoma |
| Karyotype: | (P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11 D13S317: 9 D16S539: 13,14 D5S818: 11 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 16 D3S1358: 15 D21S11: 28,30 D18S51: 12 Penta E: 10,12 Penta D: 9,13 D8S1179: 14,16 FGA: 24 CLS ∙ Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A; Rh+; HLA A2, A9, B13, B17 |
| Isoenzymes: | Me-2, 1; PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 1-2; Phenotype Frequency Product: 0.0311 |
| Products: | mucin |
References: Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. In Vitro Cell. Dev. Biol. 29A: 574-584, 1993. Chambers JA and Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105 ( Pt 2: 417-422, 1993. Hollande E et al. Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (CAPAN-1) -Relationship with differentiation. In Vitro Cell. Dev. Biol. 34:593-599, 1998. | |
