DAN-G
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
DAN-G |
Humane Pankreas Adenokarzinom Zell-Linie |
300162 |
cryovial |
265,00 |
DAN-G |
Humane Pankreas Adenokarzinom Zell-Linie |
330162 |
vital |
335,00 |
| Designation: | DAN-G |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 68 years |
| Tissue: | pancreas |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The line was derived from nude mouse xenografts initiated with cells from the tumor of a patient with cancer of the pancreas. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamin and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with calcium and magnesium free PBS, add fresh 0.025% trypsin solution for 3 to 5 minutes at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Doubling Time: | about 33 hours |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: Chu MY, Naguib FN, Iltzsch MH, el Kouni MH, Chu SH, Cha S, Calabresi P. Potentiation of 5-fluoro-2'-deoxyuridine antineoplastic activity by the uridine phosphorylase inhibitors benzylacyclouridine and benzyloxybenzylacyclouridine. Cancer Res. 44(5): 1852-1856, 1984. Knudson W, Biswas C, Toole BP. Interactions between tumor cells and fibroblasts stimulate hyaluronate synthesis. Proc Natl Acad Sci USA 81(21): 6767-71, 1984 Chu MY, Zuckerman LB, Sato S, Crabtree GW, Bogden AE, Lim MI, Klein RS. 9-Deazaadenosine - a new potent antitumor agent. Biochem Pharmacol 33: 1229-34, 1984. Feichter G, Czech W, Haag D, Goerttler K, Futtermann G, Loehrke H, Abel U. Comparison of S-phase fractions measured with flow cytometry and autoradiography in human transplant tumors. Cytometry 9(6): 605-611, 1988. Kaiser A, Brembeck FH, v. Marschall Z, Riecken EO, Wiedenmann B, Rosewicz S. Fra-1: a novel target for retinoid action. FEBS Lett 448(1):45-8, 1999. von Marschall Z, Riecken EO, Rosewicz S. Induction of matrix metalloprotease-1 gene expression by retinoic acid in the human pancreatic tumour cell line Dan-G. Br J Cancer 80(7):935-9, 1999. Klingel K, Warntges S, Bock J, Wagner CA, Sauter M, Waldegger S, Kandolf R, Lang F. Expression of cell volume-regulated kinase h-sgk in pancreatic tissue. Am J Physiol Gastrointest Liver Physiol 279(5):G998-G1002, 2000. Gutt CN, Kim ZG, Hollander D, Beuttel T, Lorenz M. CO2 environment influences the growth of cultured human cancer cells dependent on insufflation pressure. Surg Endosc. 15(3):314-8, 2001. | |
