PANC-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
PANC-1 |
Humane Pankreas Karzinom Zell-Linie |
300228 |
cryovial |
265,00 |
PANC-1 |
Humane Pankreas Karzinom Zell-Linie |
330228 |
vital |
335,00 |
| Designation: | PANC-1 |
| Depositor: | T. Lindl |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 56 years; ducts |
| Gender: | male |
| Tissue: | epithelioid carcinoma; pancreas |
| Description: | Growth is inhibited by 1 unit/ml L-asparaginase. The cells will grow in soft agar. |
| Culture Medium: | Dulbecco's modified Eagle's medium supplemented with 4 mM L-glutamine, 4.5 g/L glucose, 1 mM Na-pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with EDTA (versene) solution. Add fresh 0.05%trypsin/0.02% EDTA (versene) and let the culture to sit at 37°C until cells are dispensed. Add fresh medium, remove trypsin by centrifugation, resuspend in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | modal number = 63; 3 marker chromosomes; 1 ring |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO:10,12 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 8,10 TH01: 7,8 TPOX: 8,11 vWA: 15 D3S1358: 17 D21S11: 28 D18S51: 12 Penta E: 7,14 Penta D: 14 D8S1179: 14,15 FGA: 21 |
| Isoenzymes: | G6PD, B |
References: Lieber M et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int J Cancer 15: 741-7, 1975. Wu MC et al. Mechanism of sensitivity of cultured pancreatic carcinoma to sparaginase. Int J Cancer 22: 728-33, 1978. Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res 50: 2997-3001, 1990. Guha S. et al. Neurotensin stimulates protein kinase C dependent mitogenic signaling in human pancreatic carcinoma cell line PANC-1. Cancer Res 63: 2379-87, 2003. Khanbolooki s et al. Nuclear factor-kappaB maintains TRAIL resistance in human pancreatic cancer cells. Mol Cancer Ther 5: 2251-60, 2006. Kayed H et al. Correlation of glypican-1 expression with TGF-beta, BMP, and activin receptors in pancreatic ductal adenocarcinoma. Int J Oncol 29: 1139-48, 2006. | |
