Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
C-643 |
Humane Schilddrüsenkarzinom Zell-Linie |
300298 |
cryovial |
375,00 |
C-643 |
Humane Schilddrüsenkarzinom Zell-Linie |
330298 |
vital |
465,00 |
| Designation: | C-643 |
| Depositor: | Heldin |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | anaplastic thyroid carcinoma; thyroid |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | RPMI 1640 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | Yes, in nude mice |
References: Proc-Natl-Acad-Sci USA, 1988 Dec: 85(23): 9302-6; Thyroidology.1991 Dec;3(3): 127-31; J-Endocrinol. 1996 Nov: 151(2):277-85; Cancer Res. 1997 May 1; 57(9): 1798-806; Thyroid 1997 Oct; 7(5): 713-24. (Mol-Cell-Endocrinol. 1996 Aug 9; 121(2):143-51) | |