CLS-117
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CLS-117 |
Humane Struma Sarkom Zell-Linie |
300329 |
cryovial |
375,00 |
CLS-117 |
Humane Struma Sarkom Zell-Linie |
330329 |
vital |
465,00 |
| Designation: | CLS-117 |
| Depositor: | CLS |
| Organism: | human |
| Age/Stage: | 47-year old female |
| Tissue: | sarcoma, thyroid, polymorph cells |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Description: | in vitro established from the primary sarcoma of the thyroid gland of a 47-year-old female. |
| Culture Medium: | Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C)until the cells detach (about 2-3 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | every 3 to 5 days |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
References: J.Cancer Res. Clin. Oncol. 115 (suppl.), 1989 | |
