COLO-680N
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
COLO-680N |
Humane Speiseröhrenkarzinom Zell-Linie |
300464 |
cryovial |
375,00 |
COLO-680N |
Humane Speiseröhrenkarzinom Zell-Linie |
330464 |
vital |
465,00 |
| Designation: | COLO-680N |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Black |
| Age/Stage: | 57 years |
| Gender: | female |
| Tissue: | Esophagus |
| Morphology: | epitheloid |
| Celltype: | esophageal squamous-cell carcinoma |
| Growth Properties: | monolayer |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA and incubate at 37°C until the cells detach. Add fresh medium, collect the cells and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Immunology: | Cells express BMP-6 (bone morphogenetic protein) in standard cell cultivation conditions. |
References: Raida M, Sarbia M, Clement JH, Adam S, Gabbert HE, Hoffken K. Expression, regulation and clinical significance of bone morphogenetic protein 6 in esophageal squamous-cell carcinoma. Int J Cancer. 83(1):38-44, 1999. | |
