Detroit 562
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Detroit 562 |
Humane Pharynx (Rachen) Karzinom Zell-Linie |
300399 |
cryovial |
265,00 |
Detroit 562 |
Humane Pharynx (Rachen) Karzinom Zell-Linie |
330399 |
vital |
335,00 |
| Designation: | Detroit 562 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | adult |
| Gender: | female |
| Tissue: | pharynx; from metastatic site: pleural effusion |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, sodium pyruvate (1 mM) and lactalbumin hydrolysate (0.1%), 90%; fetal bovine serum, 10%. Alternatively DMEM:F12 (1:1 mixture) supplemented with 2 mM L-glutamine and 10 % fetal bovine serum may be used. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin for 2 to three minutes, remove trypsin and let the culture stand for 5 to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma (DAPI), bacteria and fungi were negative |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Products: | keratin |
References: Peterson WD Jr, Stulberg CS, Swanborg NK, Robinson AR. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. Peterson WD Jr, Stulberg CS, Simpson WF. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol.Med. 136: 1187, 1971 Van Wamel WJ, Vandenbroucke-Grauls CM, Verhoef J, Fluit AC. The effect of culture conditions on the in-vitro adherence of methicillin-resistant Staphylococcus aureus. J Med Microbiol. Aug;47(8): 705-9.1998. Jane M. Gould , Jeffrey N. Weiser. The Inhibitory Effect of C-Reactive Protein on Bacterial Phosphorylcholine Platelet-Activating Factor Receptor-Mediated Adherence Is Blocked by Surfactant. The Journal of Infections Diseases 186: 361-371, 2002. Adam J. Ratner, Elena S. Lysenko, Marina N, Paul, and Jeffrey N. Weiser. Synergistic proinflammatory responses induced by polymicrobial colonization of epithelial surfaces. Proc Natl Acad Sci USA 102(9): 3429-3434, 2005. Romero-Steiner S, Caba J, Rajam G, Langley T, Floyd A, Johnson SE, Sampson JS, Carlone GM, Ades E. Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies. Vaccine. 2006 Feb 2. | |
