RPMI-2650
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RPMI-2650 |
Humane Nasenscheidewand Karzinom Zell-Linie |
300323 |
cryovial |
265,00 |
RPMI-2650 |
Humane Nasenscheidewand Karzinom Zell-Linie |
330323 |
vital |
335,00 |
| Designation: | RPMI 2650 |
| Depositor: | Moore stomatitis (Indiana) |
| Organism: | Homo sapiens (human) |
| Tissue: | nasal septum; pleural effusion |
| Morphology: | squamous cell carcinoma; epithelial |
| Growth Properties: | monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Minimum essential medium Eagle (Earle's BSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.25% trypsin/0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to sit at room temperature for 5 to 10 minutes until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 THO1: 6,8 TPOX: 8 vWA: 16,18 D3S1358: 17 D21S11: 28,33.2 D18S51: 16 Penta E: 11,19 Penta D: 9,10 D8S1179: 9,13 FGA: 23,25 CLS ∙ Cell Lines Service, 2010. |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Products: | mucoid; keratin |
References: Moorhead PS. Human tumor cell line with a quasi-diploid karyotype. Exp Cell Res 39: 190-6, 1965. Hamilton TA et al. Identification of transferrin receptors on the surface of human cultured cells. Proc Natl Acad Sci USA 76: 6406-10, 1979. Rundell JO et al. Species specificity of guinea pig and human lymphotoxin colony inhibitory activity. Immunopharmacology 3: 9-18, 1981. Kornberg L. Ad-fRNK and Ad-p53 cooperate to augement drug-induced death of a transformed cell line. Anticancer Res 26: 3025-31, 2006. Tsai WC et al. Cyclooxygenase-2 is involved in S100A2-mediated tumor suppression in squamous cell carcinoma. Mol Cancer Res 4: 539-47, 2006. | |
