Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-1736 |
Humane Schilddrüsenkarzinom Zell-Linie |
300453 |
cryovial |
375,00 |
SW-1736 |
Humane Schilddrüsenkarzinom Zell-Linie |
330453 |
vital |
465,00 |
| Designation: | SW-1736 |
| Depositor: | Heldin |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | anaplastic thyroid carcinoma; thyroid |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | RPMI 1640 medium, 80%; fetal bovine serum, 20% |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
References: Heldin NE et al. The molecular biology of the human anaplastic thyroid carcinoma cell. Thyroidology 3: 127-31, 1991. Aust G et al. Differential regulation od granulocyte-macrophage colony-stimulating factor mRNA and protein expression in human thyrocytes and thyroid-derived fibroblasts by interleukin-1 alpha and tumor necrosis factor-alpha. J Endocrinol 151: 277-85, 1996. Aust G et al. CD97: a dedifferentiation marker in human thyroid carcinogenes. Cancer Res 57: 1798-806, 1997. Aust G et al. Human thyroid carcinoma cell lines and normal thyocytes: expression and regulation of matrix metalloproteinase-1 and tissue matrix metalloproteinase inhibitor-1 messenger-RNA and protein. Thyroid 7: 713-24, 1997. Voigt W et al. Potential activity of paclitaxel, vinorelbine and gemcitabine in anaplastic thyroid carcinoma. J Cancer Res Clin Oncol 131: 585-90, 2005. Roswall P et al. 2-methoxyestradiol induces apoptosis in cultured anaplastic thyroid carcinoma ells. Thyroid 16: 143-50, 2006. | |