SW-579
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SW-579 |
Humane Schilddrüsenkarzinom Zell-Linie |
300346 |
cryovial |
265,00 |
SW-579 |
Humane Schilddrüsenkarzinom Zell-Linie |
330346 |
vital |
335,00 |
| Designation: | SW-579 |
| Depositor: | Leibovitz |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 59 years |
| Gender: | male |
| Tissue: | squamous cell carcinoma; thyroid |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum Essential medium Eagle supplemented with L-glutamine, 1% NEAA, 1 mM sodium pyruvate, and 10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C)until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, produces a grade III malignant spindle and giant cell tumor in nude mice |
| Antigen Expression: | blood type O; Rh+ |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0209 |
References: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Lin JD et al. Establishment of xenografts and cell lines from well-differntiated human thyroid carcinoma. J Surg Oncol 63: 112-8, 1996. Yang DM et al. Effects of clodronate on cancer growth and Ca2+ signaling of human thyroid carcinoma cell lines. Anticancer Res 24: 1617-23, 2004. Karadag A et al. Bone sialoprotein, matrix metalloproteinase 2, and alpha(v)beta3 integrin in osteotropic cancer cell invasion. J Natl Cancer Inst 96: 956-65, 2004. Mitsiades CS et al. Fas signaling in thyroid carcinomas is diverted from apoptosis to proliferation. Clin Cancer Res 12: 3705-12, 2006. | |
