UM-SCC-14C
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
UM-SCC-14C |
Humane Mundkarzinom Zell-Linie |
300370 |
cryovial |
375,00 |
UM-SCC-14C |
Humane Mundkarzinom Zell-Linie |
330370 |
vital |
465,00 |
| Designation: | UM-SCC-14C |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Gender: | male |
| Tissue: | Mouth |
| Morphology: | epithelial |
| Celltype: | Squamous cell carcinoma |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM:Ham's F12 (1:1, vol:vol) medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium and wash once with 0.02% EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA (versene) solution and incubate at 37°C until the cells detach. Add fresh medium, remove trypsin by centrifugation, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Reverse Transcriptase: | negativ |
| Viruses: | Negative: Sendai, Ektromelia, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler's GD VII, toolan's H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis |
| Products: | keratin |
References: Grenman R et al. Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution. Int J Cancer 44: 131-6, 1989. Braakhuis BJ et al. Importance of pharmacodynamics in the in vitro antiproliferative activity of the antifolates methotrexate and 10-ethyl-10-deazaaminopterin against human head and neck squamous cell carcinoma. Biochem Pharmacol 46: 2155-61, 1993. Ruiz van Haperen VW et al. Deoxycytidine kinase and deoxycytidine deaminase activities in human tumour xenografts. Eur J Cancer 29A:2132-7, 1993. Ruiz van Haperen VW et al. Schedule dependence of sensitivity to 2',2'-difluorodeoxycytidine (Gemcitabine) in relation to accumulation and retention of its triphosphate in solid tumour cell lines and solid tumours. Biochem Pharmacol 48: 1327-39, 1994. Kubler AC et al. [Clinical effectiveness of m-THPC-PEG in a new xenogenic animal tumor model for human squamous epithelial carcinomas]. Mund Kiefer Gesichtschir 5: 105-13, 2001. Klaassen I et al. Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines. Int J Cancer 92: 661-5, 2001. Reuther T et al. The RAG 2 mouse model for xenografted human oral squamous cell carcinoma. Contemp Top Lab Anim Sci 41: 31-5, 2002. | |
