HEp-2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
HEp-2 |
Humane Kehlkopf (Larynx) Karzinom Zell-Linie |
300397 |
cryovial |
265,00 |
HEp-2 |
Humane Kehlkopf (Larynx) Karzinom Zell-Linie |
330397 |
vital |
335,00 |
| Designation: | HEp-2 |
| Organism: | Homo sapiens (human) |
| Tissue: | HeLa contamination (Larynx) |
| Morphology: | Epithelial |
| Celltype: | Carcinoma |
| Growth Properties: | Monolayer |
| Description: | The Hep-2 cell line has been described to originate from tumours which were produced in irradiated-cortisonised weanling rats after injection of epidermoid carcinoma tissue isolated from the larynx of a 56 year old male. STR (DNA)-profiling has revealed that the HEp-2 cell line is almost identical to the HeLa cell line. |
| Culture Medium: | Minimum essential medium Eagle supplemented with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids 1.0 mM sodium pyruvate and 10% fetal bovine serum |
| Subculturing: | Remove medium, add fresh 0.025% trypsin/0.02% EDTA solution, rinse and remove trypsin. Allow flask to sit at room temperature (or at 37°C) until cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 D3S1358: 15,18 D21S11: 27, 28 D18S51: 16 Penta E: 7,17 Penta D: 8,15 D8S1179: 12,13 FGA: 18,21 CLS ∙ Cell Lines Service, 2011. |
| HeLa Markers: | yes |
| Isoenzymes: | G6PD, A |
| Reverse Transcriptase: | negative |
| Products: | Keratin (The cells are positive for keratin by immunoperoxidase staining). |
References: Toolan H. Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No.1; H.Ep. No. 2; H.Ep. No. 3; and H.Emb.Rh. No. 1. Cancer Res. 14: 660-666, 1954. Moore AE, Sabachewsky L, Toolan HW. Culture characteristics of four permanent lines of human cancer cells. Cancer Res. 15: 598-602, 1955. Black FL, Melnick JL, Reissig M. Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2). Proc. Soc. Exp. Biol. Med. 93: 107-108, 1956. Moore AE. Oncolytic properties of viruses. Tex. Rep. Biol. Med. 15: 588-599, 1957. Moore AE. Tumorigenic activity of cultures. Ann. N.Y. Acad. Sci. 76(3): 497-505, 1958. Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. Groneier M et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. | |
