Y-79
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Y-79 |
Humane Retinablastom Zell-Linie |
300382 |
cryovial |
265,00 |
Y-79 |
Humane Retinablastom Zell-Linie |
330382 |
vital |
335,00 |
| Designation: | Y-79 |
| Depositor: | Reid |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 2.5 years old |
| Gender: | female |
| Tissue: | Retina |
| Morphology: | multicellular clusters |
| Celltype: | retinoblastoma |
| Growth Properties: | suspension |
| Description: | The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | Twice per week |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes |
| Isoenzymes: | PGM1, 1; G6PD, B; ES-D, 1; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.1373 |
| Reverse Transcriptase: | negative |
References: Reid TW et al. Characteristics of an established cell line of retinoblastoma. J Natl Cancer Inst 53: 347-360, 1974. Gallie BL et al. Heterotransplantation of retinoblastoma into the athymic “nude” mouse. Invest Ophthalmol Vis Sci 16: 256-9, 1977. Saari JC et al. Cellular retinol- and retinoic acid-binding proteins in transformed mammalian cells. Invest Ophthalmol Vis Sci 17: 988-92, 1978. Wong HK et al. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J Virol 70: 332-340, 1996. Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-3531, 1997. Kansara V et al. Biotin uptake and cellular translocation in human derived retinoblastoma cell line (Y-79): a role of hSMVT system. Int J Pharm 312: 43-52, 2006. Bertolesi GE et al. Ca(v)3.1 splice variant expression during neuronal differentiation of Y-79 retinoblastoma cells. Neuroscience 141: 259-68, 2006. Usui Y et al. Expression of costimulatiory molecules on human retinoblastoma cells Y-79: functional expression of CD40 and B7H1. Invest Ophthalmol Vis Sci 47: 4607-13, 2006. | |
