A-673
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-673 |
Humane Rhabdomyosarkom Zell-Linie |
300454 |
cryovial |
265,00 |
A-673 |
Humane Rhabdomyosarkom Zell-Linie |
330454 |
vital |
335,00 |
| Designation: | A-673 |
| Depositor: | Aaronson |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 15 years |
| Gender: | female |
| Tissue: | rhabdomyosarcoma |
| Morphology: | fibroblast |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM supplemented with 4.5 g/L glucose, 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse monolayer with 0.02% EDTA (versene). Add fresh 0.025% trypsin/0.02% EDTA (versene) solution and let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:20 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immunosuppressed mice |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 11/12 D13S317: 8/13 D16S539: 11/11 D5S818: 11/11 D7S820: 10/12 THO1: 9,3/9,3 TPOX: 8/8 vWA: 15/18 D3S1358: 15/17 D21S11: 28/29 D18S51: 13/13 Penta E: 12/12 Penta D: 11/13 D8S1179: 11/15 FGA: 20/21 CLS ∙ Cell Lines Service, 2009. |
| Virus Susceptibility: | very sensitive to human adenoviruses |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Prados J et al. Actin, tropomyosin and alpha-actinin as markers of differentiation in human rhabdomyosarcome cell lines induced with dimethyl sulfoxide. Cell Mol biol (Noisy-le-grand) 39: 525-36, 1993. Ferrari N et al. An improved RT-PCR protocol for the quantitation of human retinoic acid receptor RNA. Exp Cell Res 211: 121-6, 1994. Gaetje R et al. Ovarian cancer antigen CA 125 influences adhesion of human and mammalian cell lines in vitro. Clin Exp Obstet Gynecol 29: 34-6, 2002. Afjehi-Sadat L et al. Expression of proteasomal proteins in ten different tumor cell lines. Amino Acids 27: 129-40, 2004. | |
