TE-671
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
TE-671 |
Humane Rhabdomyosarkom Zell-Linie |
300355 |
cryovial |
265,00 |
TE-671 |
Humane Rhabdomyosarkom Zell-Linie |
330355 |
vital |
335,00 |
| Designation: | TE671 |
| Depositor: | Lichtenthaler |
| Organism: | Human |
| Ethnicity: | Caucasian |
| Age/Stage: | 6 years of age |
| Gender: | female |
| Tissue: | Muscle |
| Morphology: | epithelial |
| Celltype: | Rhabdomyosarcoma (originally identified as medulloblastoma) |
| Growth Properties: | monolayer |
| Description: | The original TE671 cell line was initiated by explant culture from biopsy fragments of a cerebellar medulloblastoma. The cell strain was isolated by picking a few of the multilayered round or polygonal cells which were interspersed in monolayers of stromal glial cells using Pasteur pipettes. Forms colonies in soft agar medium. Neural or glial elements were not observed upon examination with the electron microscope and no viruses were detected. The cells possess functional nicotinic acetylcholine receptors. No choline acetyltransferase or tyrosine hydroxylase activities are detectable. However, cytogenetic studies by Stratton et al. (1989) and Chen et al. (1989) have revealed that TE671 and RD are derivatives of the same cell line of rhabdomyosarcoma origin. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin/0.02% EDTA solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:3 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Modal Number: | 2n=47; 42% have 48 chromosomes |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/11 D13S317: 13/13 D16S539: 10/11 D5S818: 10/11 D7S820: 8/12 THO1: 9.3/9.3 TPOX: 9/9 vWA: 18/18 D3S1358: 15/17 D21S11: 28/29 D18S51: 13/13 Penta E: 12/12 Penta D: 11/13 D8S1179: 11/15 FGA: 20/21 CLS ∙ Cell Lines Service, 2009. |
| Immunology: | Vimentin+, desmin+, keratin- (MNF-116) |
| Receptors Expressed: | acetylcholine receptor; peripheral type benzodiazepine receptor |
References: McAllister RM et al. Establishment of a human medulloblastoma cell line. Int J Cancer 20: 206-12, 1977. Syapin PJ et al. Neuronal-like features of TE671 cells: Presence of a functional nicotinic cholinergic receptor. Brain Res 231: 365-77, 1982. Houchens DP et al. Human brain tumor xenografts in nude mice as a chemotherapy model. Eur J Cancer Clin Oncol 19: 799-805, 1983. Lindstrom JM. Assays for myasthenia gravis. U.S. Pat. 4,789,640. Chen TR et al. Cell identity resolved. Nature 340: 106, 1989. Stratton MR et al. Misidentified cell. Nature 337: 311-312, 1989. Stratton MR et al. Characterization of the human cell line TE671. Carcinogenesis 10: 899-905, 1989. Quentmeier H et al. Immunocytochemical analysis of cell lines derived from solid tumors. J Histochem Cytochem 49: 1369-78, 2001. Shankar S et al. Molecular imaging of alkylguanine-DNA alkyltransferase: further evaluation of radioiodinated derivatives of O6-benzylguanine. Nucl Med Biol 33: 399-407, 2006. Boneva N et al. Major pathogenic effects of anti-MuSK antibodies in myasthenia gravis. J Neuroimmunol 177: 119-31, 2006. | |
