A-204
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
A-204 |
Humane Rhabdomyosarkom Zell-Linie |
300109 |
cryovial |
265,00 |
A-204 |
Humane Rhabdomyosarkom Zell-Linie |
330109 |
vital |
335,00 |
| Designation: | A-204 |
| Depositor: | Giard |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 1 year old |
| Gender: | female |
| Tissue: | Muscle |
| Morphology: | epithelial |
| Celltype: | rhabdomyosarcoma |
| Growth Properties: | monolayer |
| Description: | The A-204 cell line was established in 1973 by D.J. Giard. |
| Culture Medium: | DMEM supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach. Add fresh medium, centrifuge to remove trypsin, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Split Ratio: | A ratio of 1:6 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Biosafety Level: | 1 |
| Tumorigenic: | In nude mice; forms small malignant tumors which are conform to embryonal rhabdomyosarcoma. |
| Karyotype: | diploidy and tetraploidy |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/13 D13S317: 11 / 12 D16S539: 11/12 D5S818: 12/12 D7S820: 8/10 THO1: 8/9,3 TPOX: 8/9 vWA: 15/17 D3S1358: 14/17 D21S11: 28/30 D18S51: 17/18 Penta E: 7/10 Penta D: 9/12 D8S1179: 13/15 FGA: 21/21 CLS ∙ Cell Lines Service, 2009. |
| Isoenzymes: | PGM3, 1; PGM1, 1; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1; G6PD, B |
References: Giard DJ et al.In vitro cultivation of human tumors:establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Veldwijk MR et al. Suicide gene therapy of sarcoma cell lines using recombinant adeno-associated virus 2 vectors. Cancer Gene Ther 11: 577-84, 2004. Kappler M et al. Radiosensitization, after a combined treatment of survivin siRNA and irradiation, is correlated with the activation of capases 3 and 7 in a wt-p53 sarcoma cell line, but not in a mt-p53 sarcoma cell line. Oncol Rep 13: 167-72, 2005. Mikami I et al. Efficacy of Wnt-1 monoclonal antibody in sarcoma cells. BMC Cancer 5: 53, 2005. Lee TH et al. Effect of mycelial extract of Clavicorona pyxidata on the production of amyloid beta-peptide and the inhibition of endogenous beta-secretase in vitro. J Microbiol 44: 665-70, 2006. | |
