RD
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
RD |
Humane Embryonale Rhabdomyosarkom Zell-Linie |
300401 |
cryovial |
265,00 |
RD |
Humane Embryonale Rhabdomyosarkom Zell-Linie |
330401 |
vital |
335,00 |
| Designation: | RD |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | embryo |
| Gender: | female |
| Tissue: | rhabdomyosarcoma |
| Morphology: | spindle cells and large multinucleated cells |
| Growth Properties: | monolayer |
| Description: | This line has recently been shown to be at least parental, if not identical, to TE 671 (ATCC HTB 139). |
| Culture Medium: | Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10% |
| Subculturing: | Add fresh 0.025% trypsin and place at 37°C for 3 to 5 minutes. Add fresh culture medium, aspirate and dispense into new culture vessels. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | Every 3 to 4 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Modal Number: | 2n=48 |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia |
| Products: | myoglobin; myosin ATPase |
References: McAllister RM et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-6, 1969. Gershwin ME et al. Immunobiology of heterotransplanted human tumors in nude mice. J Natl Cancer Inst 58: 1455-61, 1977. Chen TR et al. Cell identity resolved. Nature 340: 106, 1989. Stratton MR et al. Misidentified cell. Nature 337: 311-312, 1989. Hu-Lieskovan S et al. EWS-FLI1 fusion protein up-regulates critical genes in neural development and is responsible for the observed phenotype of Ewing's family of tumors. Cancer Res 65: 4633-44, 2005. Mercer SE et al. Mirk/Dyrk1b mediates cell survival in rhabdomyosarcomas. Cancer Res 66: 5143-50, 2006. | |
