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Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Yac-1 |
Murine Lymphom Zell-Linie |
400383 |
cryovial |
265,00 |
Yac-1 |
Murine Lymphom Zell-Linie |
440383 |
vital |
335,00 |
| Designation: | YAC-1 |
| Depositor: | R. Kiessling |
| Organism: | Mus musculus (mouse) |
| Strain: | A/Sn |
| Tissue: | lymphoma |
| Celltype: | lymphoblast |
| Growth Properties: | suspension |
| Description: | Moloney murine leukemia virus (Mo-MuLV) induced lymphoma. The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/ml and maintain between 2 x 105 and 2 x 106 cells/ml. |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Biosafety Level: | 1 |
References: Cikes M et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J Natl Cancer Inst 50: 347-362, 1973. Kiessling R et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur J Immunol 5: 112-117, 1975. Kiessling R et al. Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non-immunized semi-syngeneic mice against a mouse lymphoma line. Int J Cancer 15: 933-40, 1975. Okazaki IJ et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J Biol Chem 271: 22052-22057, 1996. Liao HF et al. Effect of a periodized exercise training and active recovery program on antitumor activity and development of dendritic cells. J Sports Med Phys Fitness 46: 307-14, 2006. Boyadjieva N et al. Role of beta-endorphin, corticotropin-releasing hormone, and autonomic nervous system in mediation of the effect of chronic ethanol on natural killer cell cytolytic activity. Alcohol Clin Exp Res 30: 1761-7, 2006. | |