Gerne beantworten wir Ihre Fragen
rund um unsere Zell-Linien
und weitere Produkte.
Tel.: +49 (0)6221 700799
Fax.: +49 (0)6221 700717
WEHI-164
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
WEHI-164 |
Murine Fibrosarkom Zell-Linie |
400438 |
cryovial |
265,00 |
WEHI-164 |
Murine Fibrosarkom Zell-Linie |
440438 |
vital |
335,00 |
| Designation: | WEHI-164 |
| Depositor: | Warner |
| Organism: | Mus musculus (mouse) |
| Strain: | BALB/c |
| Tissue: | fibrosarcoma; (methylcholanthrene induced) |
| Morphology: | fibroblast |
| Growth Properties: | adherent |
| Description: | This line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene. After pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin. They can be used as target cells for short term chromium release cytotoxicity assays. Tested and found negative for ectromelia virus (mousepox). |
| Culture Medium: | RPMI 1640 medium, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution for 1 to 2 minutes. Remove trypsin and place the flask at 37°C until the cells detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:20 is recommended |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in Balb/c mice |
References: Rollinghoff M et al. Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells. Proc Soc Exp Biol Med 144: 813-818, 1973. Lust JA et al. Heterogeneity of natural killer cells in the mouse. J Exp Med 154: 306-17, 1981. Carbone G et al. In vitro detection of cell-mediated immunity to individual tumor-specific antigens of chemically induced BALB/c fibrosarcomas. Int J Cancer 31: 483-9, 1983. Ziegler-Heitbrock HW et al. A rapid assay for cytotoxicity of unstimulated human monocytes. J Natl Cancer Inst 72: 23-29, 1984. Ziegler-Heitbrock HW et al. Killer cell activity of human monoblastic leukemia cells as detected with a monocyte-specific target cell. Blood 65: 8-14, 1985. Sugarman BJ et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. Knowlton KU et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J Virol 70: 7811-7818, 1996. Panthel K et al. Prophylactic anti-tumor immunity against a murine fibrosarcoma tiggered by the Salmonella type III secretion system. Microbes Infect 8: 2539-46, 2006. Cioffi G et al. Antiproliferative triterpene saponins from Entada africana. J Nat Prod 69: 1323-9, 2006. | |
